Objective: To immobilize the permeabilized baker’s yeast using calcium alginate beads and establish the characteristic kinetic parameters of immobilized enzyme in column reactor.
Theory: Enzymes are unique biocatalyst, which accelerate certain enzyme substrate reactions at extremely mild environmental conditions (pH, Temperature etc) to produce important metabolites which are otherwise produced by chemical reactions at higher temperature and pressure. In actual practice microbes are normally cultivated in the batch cultivation mode which produces enzymes in intra/extracellular manner. These enzymes react with specific substrate(s) & produce interesting metabolites. After the cultivation, products are recovered and microbial cells (containing enzymes) are discarded. This is a wasteful procedure, instead it will help if the enzymes (or whole cells) are some-how separated and reused repeatedly to economize the process. Immobilization features attachment of enzymes (or whole cells) on insoluble supports or entrapment with-in the gel/membrane so that it may be separated after its reaction with the substrate and reused several times for metabolite production.