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Estimation of growth kinetic parameters in batch fermentation
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Objective: To study the batch growth kinetics and establish the key kinetic parameters

a)    Maximum specific growth rate (μm) h -1

b)    Saturation constant, (Ks) g.L-1

c)    Overall yield of biomass (Yx/s) g cell. g-1 substrate

d)    Dynamic yield (Yx/s) g cell. g-1 substrate

e)    Overall productivity of biomass, g cell. 1-1.h-1

 
Culture Medium:

 

S. No.

Medium Component

Concentration (g.L-1)

1.

Glucose

3.0

2.

Ammonuim sulphate

2.5

3.

Na2HPO4

7.9

4.

KH2PO4

3.7

5.

MgSO4, 7 H2O

0.625

6.

NaCl

0.625

7.

CaCl2

0.019

8.

Yeast extract

0.125

 

 

Prepare 400 ml seed culture medium of the above composition. Take 200 ml medium each in two 500 ml flasks. Adjust the pH to 7.0 with NaOH. A mixture containing Glucose and CaCl2 is to be taken in one flask while the remaining medium components are taken in another flask. The above two flasks are sterilized separately and reconstituted in laminar hood only after cooling.

 

Acid/Base Solutions for pH control: Prepare 200 ml each of 1N HCl and 1N NaOH solutions in 500 ml flasks. Place cotton plugs and connect the silicon tubing for peristaltic pumps. Clamp the tubings and autoclave them.

 

Antifoam agent: Prepare 25 ml of a 5% suspension of silicon oil in poly propylene glycol (PPG) solution and sterilize in the autoclave.

 

Production Medium: The above medium recipe composition is also to be used for the bioreactor.

  

 

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