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Simultaneous measurement of specific growth / death rate of micro organisms


Objective:  To determine the specific thermal death rate of S. cerevisiae at different temperature in a batch cultivation.



Theory:  The killing of the microbial population by heat (elevated temperatures, dry / moist heat etc) is majorly applied in fermentation laboratories &/or industries. In all the above procedures the destruction or death of the microorganisms by heat features loss of viability, not destruction in physical sense. The physical entity (dead cells) of the microorganism will remain in the bioreactor and will still contribute in the optical density values of different samples from the bioreactor. The cell death rate by heat at a specific temperature follows a monomolecular rate of reaction as shown below:


Where "NV" is the number of viable cells


"kd" is the specific thermal death rate at temperature "T".



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